Three-way interactions among the Sfh, StpA and H-NS nucleoid-structuring proteins of Shigella flexneri 2a strain 2457T
نویسندگان
چکیده
منابع مشابه
H-NS, Its Family Members and Their Regulation of Virulence Genes in Shigella Species
The histone-like nucleoid structuring protein (H-NS) has played a key role in shaping the evolution of Shigella spp., and provides the backdrop to the regulatory cascade that controls virulence by silencing many genes found on the large virulence plasmid. H-NS and its paralogue StpA are present in all four Shigella spp., but a second H-NS paralogue, Sfh, is found in the Shigella flexneri type s...
متن کاملAn H-NS Family Protein, Sfh, Regulates Acid Resistance by Inhibition of Glutamate Decarboxylase Expression in Shigella flexneri 2457T
The glutamate-dependent acid-resistance system is the most effective acid tolerance pathway in Shigella, allowing survival in extremely acidic environments. However, the regulation of this system in Shigella remains elusive. In the current study, we identified significant differences in the levels of glutamate decarboxylase between three Shigella flexneri strains with different levels of acid r...
متن کاملComplete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T.
We determined the complete genome sequence of Shigella flexneri serotype 2a strain 2457T (4,599,354 bp). Shigella species cause >1 million deaths per year from dysentery and diarrhea and have a lifestyle that is markedly different from those of closely related bacteria, including Escherichia coli. The genome exhibits the backbone and island mosaic structure of E. coli pathogens, albeit with muc...
متن کاملReciprocal transcriptional and posttranscriptional growth-phase-dependent expression of sfh, a gene that encodes a paralogue of the nucleoid-associated protein H-NS.
The IncHI1 self-transmissible plasmid pSf-R27 from Shigella flexneri 2a strain 2457T harbors sfh, a gene that codes for a protein with strong amino acid sequence homology to the global transcription regulator and nucleoid-associated protein H-NS and to its paralogue, StpA. Previously, we discovered that the expression of sfh mRNA is growth phase dependent such that in cultures growing in Lennox...
متن کاملHeteromeric interactions among nucleoid-associated bacterial proteins: localization of StpA-stabilizing regions in H-NS of Escherichia coli.
The nucleoid-associated proteins H-NS and StpA in Escherichia coli bind DNA as oligomers and are implicated in gene regulatory systems. There is evidence for both homomeric and heteromeric H-NS-StpA complexes. The two proteins show differential turnover, and StpA was previously found to be subject to protease-mediated degradation by the Lon protease. We investigated which regions of the H-NS pr...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2003
ISSN: 0950-382X,1365-2958
DOI: 10.1046/j.1365-2958.2003.03515.x